Revue internationale de recherche et développement en pharmacie et sciences de la vie

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VALIDATED RP-HPLC METHOD AS A TOOL FOR THE ESTIMATION OF TINIDAZOLE IN PHARMACEUTICAL DOSAGE FORMS

P. Deepika, R. Suthakaran

A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of Tinidazole in both pure and pharmaceutical dosage forms. . Chromatographic separation achieved isocratically on a ODS2 reverse phase column [Use Symmetry C18, 250 X 4.6mm, 5μ] utilizing a mobile phase of Methanol. The flow rate was 0.5 ml/min and the effluents were monitored at 310 nm. The retention time was 4.610min. The linearity was in the range of 5-25μg / ml. This method was validated for linearity, precision and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.