Journal de pharmacocinétique et thérapeutique expérimentale

Abstrait

Quantification of Antiplatelet Effects through Ex Vivo Platelet Aggregation Assays

Linsheng Zang

The quantification of antiplatelet effects utilizing ex vivo platelet aggregation assays. Platelet aggregation plays a crucial role in hemostasis and thrombosis, making it a key target for assessing the efficacy of antiplatelet therapies. Various agents have been developed to modulate platelet function and reduce the risk of thrombotic events. To accurately evaluate their effects, ex vivo assays offer a controlled environment that mimics physiological conditions. In this investigation, a comprehensive review of the principles behind ex vivo platelet aggregation assays is provided, outlining their relevance in assessing the pharmacological activity of antiplatelet agents. The methodologies involved in preparing platelet-rich plasma and conducting aggregation assays are discussed in detail. Furthermore, the study examines the factors influencing assay results, including platelet concentration, agonist selection, and assay conditions. Through a series of experiments utilizing established antiplatelet agents, the utility of ex vivo platelet aggregation assays is demonstrated. The results underscore the sensitivity of these assays in detecting differences in platelet aggregation patterns, thus highlighting their potential for guiding therapeutic decisions. The study also addresses the limitations of these assays, such as their reliance on blood samples and potential variability. In conclusion, this paper sheds light on the significance of ex vivo platelet aggregation assays as a valuable tool for quantifying the antiplatelet effects of various therapeutic agents. By enhancing our understanding of platelet function modulation, these assays contribute to the advancement of personalized medicine in the realm of cardiovascular health and thrombosis prevention.