Journal de pharmacocinétique et thérapeutique expérimentale

Abstrait

IMMUNOMODULATING PROPERTIES OF A BIOLOGICAL PRODUCT BASED ON HORSE PLACENTA

Fakhradiyev Ildar,

Currently, a fairly wide range of studies is devoted to studying the possibilities of using placenta extracts obtained by lysis of human placental tissues, sheep, goats, cattle and horses in medicine.

Based on these data, purpose to our study was to explore ix biological properties of the active drug based on horse placenta under conditions in vitro.

Research methods: percoll density gradient isopycnic centrifugation, trypan blue exclusion test, MTT test, cell culture, light microscopy, flow immuno-cytofluorimetry, enzyme-linked immunosorbent assay, proliferation test using intravital fluorescent CFSE stain. Bytes iopreparat s based horse placenta no cytotoxic th action on human mononuclear cells of peripheral blood at 24 hours exposure to concentrations not exceeding 3,250 pg / ml. and have a cytoprotective effect against mononuclear cells in human peripheral blood, reducing the intracellular level of reactive oxygen species after short-term exposure.