Biologie Cellulaire et Moléculaire

Abstrait

The Prognostic Value and Biological Function of Microrna-491-5p for Gastric Cancer

Pan-Quan Luo, Li-Xiang Zhang, Bai-Cheng Ding and A-man Xu

Background: Gastric Cancer (GC) is one of the deadliest cancers in China. And, it can be regulated by MicroRNAs (miRNAs) generally. miR-491-5p function as a tumor suppressor in different types of cancer, but we still don’t know the role of miR-491-5p in GC.

Methods: RT-PCR was used to detect the level of miR491-5p and its clinical vale, functional experiments including CCK-8 assay and transwell assay were performed. Furthermore, the underlying mechanism was explored through qRT-PCR and western blotting. The function of miR-491-5p was also identified in vivo.

Results: miR-491-5p was related with TNM stage, patients with high miR-491-5p had better prognosis. We found that high level of miR-491-5p caused a weak cell proliferation, migration and invasion abilities. In order to explore the role of miR-491-5p in vivo, we set a xenograft mouse model, and found that high level of miR-491-5p suppressed tumor growth. Moreover, we found that miR-491-5p regulate the tumor development thought regulate the expression of EMT (Epithelial-Mesenchymal Transition), cell adhesion genes and IFITM2.

Conclusions: miR491-5p is an important prognostic bio-marker, which can offer help for diagnosis and treat of GC patients, and miR-491-5p function as a tumor suppressor in GC both in vitro and in vivo.