Journal de médecine vétérinaire et de santé

Abstrait

Seroprevalence of Brucellosis through Different Tests (Rose Bengal Plate, Serum Agglutination, ELISA)

Laila Jamil

Brucellosis is a highly contagious, zoonotic and economically important bacterial disease of animals worldwide. The present study was undertaken to detect the Brucella abortus antibodies in serum. Antibodies usually begin to appear in the blood at the end of the first week of the disease. RBPT (Rose Bengal Plate test) is of value as a screening test especially in high risk rural areas where it is not possible to perform SAT (Serum Agglutination Test) and ELISA. In this study RBPT was used as a screening test in conjunction with SAT and ELISA. Twenty eight positive Punjab samples were obtained out of 800 through initial screening by RBPT (seroprevalence=3.5%). Serum that give positive results were confirmed by more specific serological tests SAT and ELISA. Through SAT the positive percentage obtained was 40% out of 20 samples. ELISA yields higher sensitivity and specificity. A total of 44 (RBPT positive and suspected) samples were tested out of which 13 proved to be positive (Seroprevalence=29.54%). Serological reactions were all based on the detection of anti-LPS antibodies. Higher prevalence was found in buffaloes than cattle.