Maladies infectieuses cliniques et pratique

Abstrait

Nosocomial Contamination and Bacterial Resistance in Intensive Care Units after Disinfection: An Emerging Challenge under Biofilm and Planktonic Growth Condition

Luciana Debortoli de Carvalho, Paloma Ohana S Abreu, Julio Lenin Diaz Guzman, Camila Pacheco S M Da Mata, Cid Edson Mendonca Povoas, Ana Carolina Morais Apolonio, Vinicius Novaes Rocha Guerra, Valeria Ferreira Fernandes, Uener Ribeiro Dos Santos, Aline Silva, Renato Fontana, Joao Luciano Andrioli, Raynah Neves Da Hora, Heitor P Filho, Ana Paula M L De Lemos, Maria Silvana Alves, Rachel Passos Rezende, Guilherme Rosemberg Guimaraes Queiroz, Joao Carlos Teixeira Dias, Ana Paula Trovatti Uetanabaro, Carla Cristina Romano, Aline Oliveira Da Conceicao

Disinfectants are widely used in healthcare facilities to prevent the occurrence of hospital associated infections (HAIs), especially in intensive care units (ICUs). Microorganisms adhere to different surfaces and become physically structured in biofilms by secreting extracellular polymeric substances (EPSs) which act as an external shield for the microorganisms, reducing the penetration and diffusion of biocidal substances. This study analyzed the presence of phenotypically resistant Gram-negative rods in ICUs and investigated whether biofilms are responsible for the persistence of multidrug-resistant bacteria in these specialist hospital wards. Multidrug-resistant Gram-negative rods were isolated after ICU disinfection and evaluated for susceptibility to three disinfectants [benzalkonium chloride and biguanide (BCB), sodium hypochlorite (NaClO), and hydrogen peroxide (H2O2)] under two different conditions: when they are in a planktonic state (unicellular-life phase) and after induction to form biofilms (multicellular-life phase). We compared the efficacy of these chemical disinfectants in removing monospecies biofilms by quantifying the mature biofilm biomass using the crystal violet technique, and through evaluation using scanning electron microscopy (SEM).All disinfectants tested showed bactericidal activity against bacteria growing in suspension. After inducing the bacteria to form a biofilm, we observed that BCB was unable to reduce biofilm biomass, while H2O2 was only weakly effective against A. baumannii, A. calcoaceticus complexes ACB (p<0.05) and E. coli ATCC 25922 (p<0.05). SEM images corroborated crystal violet data regarding the pronounced NaClO activity observed against all bacteria (p<0.0001) and allowed a better evaluation of the action of BCB, demonstrating variation in sensitivity to this disinfectant for each species evaluated. Multidrug-resistant bacteria isolated from ICU inanimate surfaces did not show the same sensitivity to the assessed disinfectants when they were in a biofilm as when they were in a single cell state, and it appeared that biofilms are able to reduce the activity of disinfectants and contribute to the spread of bacteria involved in the occurrence of HAIs.