Biologie Cellulaire et Moléculaire

Abstrait

NGF and CNTF Expression, Regulation Mechanism by miRNA in Acute Paralytic Strabismus

Hua Liu, Nian Tan, Duo Xu, Chong-Yi Li and Guang-Jun Xian

Nerve growth factor (NGF) and ciliary neurotrophic factor (CNTF) are well known neurotrophic factors and widely used in the clinical treatment for its promotion effect on peripheral nerve regeneration. And they were also recommended for the acute paralytic strabismus treatment. However, whether the NGF and CNTF have protective effect for the extraocular muscles of acute paralytic strabismus patients is still poorly understood. Thus, in this study, we want to evaluate the biological function of NGF and CNTF on the extraocular muscle cells and revealed the regulation mechanism behind it. Firstly, the relative expression of ngf and cntf was assessed by Quantitative Realtime Polymerase Chain Reaction (RT-PCR). Then, the influence of NGF and CNTF on the extraocular muscle cell proliferation was determined by Cell Counting Kit-8 (CCK-8). The inflammatory response in muscle cells after NGF and CNTF treatment was evaluated by ELISA and Reactive Oxygen Species (ROS) detection. In addition to this, the up-stream regulation of the ngf and cntf expression was also studied. The TargetScan was used for the predication of potential miRNAs targeting with ngf and cntf 3’-UTR, which is soon confirmed by luciferase activity assay. Taken together, all the results above indicated that NGF and CNTF could promote the muscle cells proliferation and inhibit the inflammatory levels, then exert protective effect on the muscle cells function. It was conceivable that let 7-5p was the up-stream regulator of ngf and cntf, and let 7-5p might serve as a potential molecular target for acute paralytic strabismus treatment.