Biologie Cellulaire et Moléculaire

Abstrait

Guben Xiezhuo Recipe Alleviated Colon Dialysis on Urotoxin-Induced Intestinal Barrier Injury and Systemic Inflammation

Haidong He, Hongmei Huan, Yuyan Tang, Hongru Wang, Weiqian Sun, Xudong Xu

Background: Chronic kidney disease (CKD) is a major disease that endangers public health around the world. The urinary toxins produced in the disease can induce intestinal barrier damage and systemic inflammation, which further aggravates the disease and creates a vicious circle. Therefore, this work carried out cell-level research to determine the ability of Guben Xiezhuo Recipe for colon dialysis to repair the intestinal barrier and to interfere with the entire process.

Methods: T84 cells were divided into six groups, one of which was a control group, and the experimental components were divided into five groups according to the level of urinary toxin content and the different drugs added. Perform a series of tests on six groups. Immunofluorescence technique was used to analyze the expression characteristics of intestinal epithelial tight junction protein Occludin in intestinal epithelial cells in the intestinal barrier injury model. Western Blot technology was used to analyze the expression of ZO-1 and Cluadin-1 in intestinal epithelial cells in the intestinal barrier injury model. RT-PCR technology was used to analyze the expression changes of Keap1, Nrf2, ROS, and NF-κB mRNA in intestinal epithelial cells. ELISA was used to analyze the content of inflammatory signal hypersensitive C-reactive protein and interleukin-6 (IL-6) in cell culture fluid.

Results: The results of immunofluorescence analysis and Western Blot analysis demonstrated the ability of urinary toxins produced by CKD to destroy the intestinal barrier. The results of RT-PCR analysis explained the mechanism of urinary toxins destroying the intestinal barrier from the molecular biology level. The results of ELISA analysis indicate that the intestinal barrier damage will further induce systemic inflammation. Through the comparison of the above analysis results in the six groups, it can be shown that Guben Xiezhuo Colon Dialysis Recipe has an excellent therapeutic effect on intestinal epithelial injury.

Conclusion: Through the detection and comparison of the contents of intestinal barrier-related proteins and inflammatory signal factors in each group, it is proved that the Guben Xiezhuo Recipe colon dialysis has an excellent therapeutic effect on the repair of intestinal barrier damage and further inhibits the deterioration of CKD, which also provides an optional auxiliary strategy