Journal de biochimie et de biologie cellulaire

Abstrait

Gene Expression of Human Lung Adenocarcinoma-Derived A549 Clones

Sadhwy S

For clarifying interactions between genes and proteins, the GenSensor Suite comprises of four web tools. The outcomes of GenPath reveal whether categories of biochemical, regulatory, or other gene set are over- or underrepresented in an input list when compared to a background list. GenPath includes some specific gene sets in addition to all common gene sets that can be searched. Users may include unique background lists. GenInteract creates an interaction gene list from a single input gene and then uses GenPath to evaluate it. In order to extract a gene list from a list of PubMed IDs and query it in GenPath, GenPubMed employs a PubMed query to find the list of PubMed IDs. Users of GenViewer can compare one gene set to another in GenPath. For healing harmed or irreparable lung tissue, stem cell treatment looks to hold promise. However, because the molecular mechanism underlying differentiation of alveolar epithelial cells is not entirely known, developing a straightforward and repeatable methodology for lung progenitor populations is challenging. Using the human alveolar epithelial type II cell line A549, we looked into an in vitro system to examine the control mechanisms of alveolus-specific gene expression. A549 subpopulations were cloned, and each clone was divided into five categories based on the cell morphology and marker gene expression. B7 and H12, two clones, underwent additional examination. Surfactant protein C, an ATII marker, was increased in both H12 and B7 when cultured in a serum-free environment. An ATI marker known as aquaporin 5 (AQP5) was highly elevated in B7 and H12, respectively.