Biologie Cellulaire et Moléculaire

Abstrait

Exploration on the Clinical Effect of Different Autotransfusion Methods on Patients with Femoral Shaft Fracture Surgery

Huan Wang, Dan Yin, Jinhuo Wang, Zhenzhou Li, Jiaming Xu, Ziwei Zhang, Xiaoxiao Wang, Yiqun Kang, Jianrong Guo

Objective: To explore the clinical effect of predeposit, salvage and hemodilution autotransfusion on patients with Femoral Shaft Fractdure (FSF) surgery. Methods: Selected FSF patients were randomly divided into 3 groups: intraoperative blood salvage autotransfusion group, preoperative hemodilution autohemotransfusion group and predeposit autotransfusion. After the operation for 5 days, body temperature, heart rate, blood platelet (PLT) and Hemoglobin (Hb) of patients were determined; the concentrations of EPO and G-MSF in the 3 groups were calculated by ELISA assay; the content of CD14+ monocytes were calculated by FCM assay. The growth time and condition of the patient’s callus were determined at the 30^th, 45^th and 60^th days after operation. Cox regression analysis was used to analyze the correlation between EP0, G-MSF, CD14+ mononuclear content, callus growth and autotransfusion methods.

Results: There were no statistically significant differences in body temperature and heart rate between the 3 groups (P >0.05). PLT and Hb in Predeposit group were markedly increased compared to that in Salvage group and Hemodilution group. The concentrations of EPO and G-MSF in Predeposit group were markedly increased compared to that in Salvage group and Hemodilution group. The content of CD14+ monocytes in Predeposit group was significantly higher compared to that in Salvage group and Hemodilution group. Predeposit autotransfusion promotes callus growth more quickly. The concentration of EP0 and G-MSF were positively correlated with callus growth in the three groups, and the content of CD14+ monocytes was not correlated in any of the three groups.

Conclusion: Predeposit autotransfusion promoted the recovery of FSF patients after operation more quickly compared to salvage autotransfusion and hemodilution autotransfusion.