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Effects of Putrescine on Anti-Oxidative Enzymes in Two Rice Cultivars Subjected To Salinity

Nirmalya Ghosh and Malay Kumar Adak

A study was conducted for elucidation of expression of isozymic profiles with respect to salinity along with putrescine application in two rice varieties cv. Nonabokra and cv. Swarna at 200mM NaCl alone and with 2mM putrescine. Preliminarily these two varieties displays differential pattern of accumulation of Na+ as revealed from SEM micrograph studies. There recorded significant variation in activities in-vitro as well as by in-gel studies of Guaiacol Peroxidase (GPX), Ascorbate Peroxidase (APX), Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Reductase (GR) enzymes. Activities for GPX, APX and SOD followed significant up regulation under salinity. In contrary CAT and GR enzymes were subdued in both varieties. Putrescine improved the activity for SOD, GPX and APX. CAT and GR maintained stable activity with putrescine. A number of isozymic bands were found with the induction of salinity and putrescine treatment. For SOD three distinct bands were recorded as Cu/Zn/Mn-Fe SOD. For GPX and APX multiple bands were revealed in activity gel. On the contrary CAT was insensitive with the putrescine induction and hardly there recorded any variation. GR isozyme was more prominent in band intensities both in salinity and putrescine. Conclusively different isozymic profiles have contributed to resist salinity along with putrescine.