Journal of Bioremediation & Biodegradation

Abstrait

Effects of Growth Regulators and Sucrose on in vitro Nodal Segments and Shoot Tip Culture of Jojoba (Simmondsia chinensis (Link) Genotypes

Amr SM Mohamed, Magdi AA Mousa and Ahmed AS Bakhashwain

The responses of four jojoba genotypes to in vitro nodal segments and shoot tip culture under different growth regulators combinations and sucrose concentrations were investigated. All experiments were laid out in Completely Randomized Blocks (CR) design using 5 replicates. The results revealed that the jojoba genotype ‘Al-Maddenah’ conveyed the highest response to nodal segments, with MS culture medium supplemented with BAP (3 mg) alone and BAP (3 mg), in combination with IAA (7 mg). Modifying sucrose concentration in the culture medium (20 g, 40 g, 50 g and 60 g) enhanced shoot formation of nodal segments of the genotype ‘Hada Al-Sham”. Concerning shoot tip culture, explants of the genotype ‘Hada Al-Sham’ were cultured on MS+BAP (5 mg)+IAA (5 mg) produced the highest percentages of bud sprouting and shoot formation. Increasing sucrose concentration from 20 g to 60 allowed the genotype ‘Hail-B’ to produced the highest percentage of bud sprouting and shoot formation.