Biologie Cellulaire et Moléculaire

Abstrait

Effects and Mechanisms of Trifluridine alone or in Combination with Cryptotanshinone in Inhibiting Malignant Biological Behavior of Gastric Cancer

Richard Young

Background: Gastric cancer(GC) is the fourth most common malignant tumor worldwide. This study aimed to investigate the effect of a combination of cryptotanshinone (CTS) and trifluorothymidine (FTD) on GC.

Methods: The effect of the combined or separate use of FTD and CTS on HGC-27 and AGS cells was detected using the CCK8 assay. The combined index of FTD and CTS was calculated using Compusyn software. Additionally, we applied flow cytometry to study the cell cycle and apoptosis and investigated the amount of FTD incorporated into DNA by immunofluorescence assay. Protein expression was monitored by western blotting (WB). Furthermore, the effect of TAS-102 in combination with CTS was studied in a xenograft nude mouse model.

Conclusions: FTD combined with CTS has a synergistic anti-gastric cancer effect in vitro and in vivo and is a promising treatment option for advanced GC.