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Diagnostic of Invasive Pulmonary Aspergillosis Using Nested PCR and Culture

Hedhili S, Souid H, Baccouchi N, Jemli B and Gargouri S

A total of 120 pulmonary samples collected from 100 patients who were at high risk for invasive aspergillosis were examined by mycological test and nested PCR specific of A. fumigatus and A. flavus. Nested PCR was done using two sets of primers alp11/alp12 and alp13/alp14 coding for alkaline protease. Nineteen seven of these patients had negative PCR and culture results, two patients had nested PCR+/culture- PCR products revealed the presence of A. flavus. Thus only one patient had culture+/PCR-, morphologic identification characterized an A. niger. From 100 patients, only 3 had probable invasive pulmonary aspergillosis. Nested PCR and fungal culture correlate to 97% of case. Our data suggest that this nested PCR method may confirm and can improve the diagnosis of IPA in highrisk patients.