Journal of Bioremediation & Biodegradation

Abstrait

Degradation of Phenol by Mixed Culture of Locally Isolated Pseudomonas Species

Sadia Afrin Jame, A.K.M. Rashidul Alam, A.N.M. Fakhruddin and Md. Khorshed Alam

Textile, pharmaceuticals and automobile waste most often contain phenolic wastes. An attempt was made to degrade phenol using locally isolated bacteria. Moreover, as in nature pure culture is rarely found rather than mixed culture, therefore, attempt was also made to improve the degradation using mixed culture of Pseudomonas species. All isolates could completely degrade phenol up to 600 ppm. Isolate Pseudomonas FA degraded 800 ppm phenol completely in 72 hours, but the isolates Pseudomonas SA, TK and KA degraded only 39.33, 43.83 and 33.16% of 800 ppm phenol respectively in 96 hours. Complete removal time was also shorter for the isolate Pseudomonas FA compares to the other isolates. Patterns of growth were similar for all of the isolates, but maximum growth was found with the isolate FA on 600 ppm phenol. Complete degradation time was decreased with mixed culture and removal rate of phenol was 25 ppm/h in mixed culture of all combinations and was higher than that of the single culture of the isolates. In mixed culture study, the growth of bacteria was also increased.