Notre groupe organise plus de 3 000 séries de conférences Événements chaque année aux États-Unis, en Europe et en Europe. Asie avec le soutien de 1 000 autres Sociétés scientifiques et publie plus de 700 Open Access Revues qui contiennent plus de 50 000 personnalités éminentes, des scientifiques réputés en tant que membres du comité de rédaction.
Les revues en libre accès gagnent plus de lecteurs et de citations
700 revues et 15 000 000 de lecteurs Chaque revue attire plus de 25 000 lecteurs
Sana Mustafa, Wajiha Hashim, Saima Khaliq, Azizuddin and Rashid Ali Khan
IBrain functions can be affected by the dietary precursors of neurotransmitters. Tryptophan (TRP) is the precursor of serotonin (5- Hydroxytryptamine; 5-HT) neurotransmitter that plays an important role in sleeping, mood, eating behavior, consciousness and cognitive functions. Present study describes the estimation of tryptophan in rat brain by aqueous-reverse-phase high performance liquid chromatography. Estimation was carried out on a shim pack end-capped C-18 column using water as mobile phase (pH=9 adjusted by addition of 0.1 mM NaOH) at a flow rate of 1 ml min-1. Eluents were monitored at 273 nm by an ultra-violet detector. The method was linear (R2=0.9973) and the limit of detection (LOD) and limit of quantification (LOQ) were 4.3920 and 13.3091 ng ml-1, respectively. The developed HPLC method is simple convenient, reliable and economical for the estimation of tryptophan in rat brain. In the present study, Albino Wistar male rats were randomly divided into two groups control and seaweeds treated rats. Seaweeds (Jolyna Laminiriodes) were orally given to test rats at a dose of 200 mg kg-1 whereas control rats were given vehicle for 28 days. After 28 days of treatment, rats were decapitated and its brain samples were taken out for the estimation of TRP. Current study observed increased in TRP levels in the brain samples of seaweed treated rats as compared to control rats. Therefore present study suggested the use of seaweeds in the treatment of disorders caused by TRP depletion. By this newly developed method for the estimation of TRP, effect of various drugs on TRP levels in the brain could be studied.